Entry information : MsPrx15 (pxdC)
Entry ID 277
Creation 2006-09-15 (Christophe Dunand)
Last sequence changes 2010-09-28 (Christophe Dunand)
Sequence status complete
Reviewer Nizar Fawal
Last annotation changes 2011-03-04 (Nizar Fawal)
Peroxidase information: MsPrx15 (pxdC)
Name (synonym) MsPrx15 (pxdC)
Class Class III peroxidase    [Orthogroup: Prx001]
Taxonomy Eukaryota Viridiplantae Streptophyta Fabaceae Medicago
Organism Medicago sativa (alfalfa)    [TaxId: 3879 ]
Cellular localisation N/D
Tissue types Glandular trichome
Roots
Inducer Nutrient deficiency (Al, Zn, P, K, Fe, N)
Repressor N/D
Best BLASTp hits
Perox score E-value MsPrx15
start..stop
S start..stop
MtPrx15 689 0 4..353 3..352
GmPrx260 563 0 20..352 15..348
GmPrx69 558 0 20..352 14..346
LjPrx09 551 0 7..352 4..349
Literature and cross-references MsPrx15 (pxdC)
Literature REFERENCE 1 Abrahams,S.L., Hayes,C.M. and Watson,J.M. Organ-specific expression of three peroxidase-encoding cDNAs from lucerne (Medicago sativa).
REFERENCE 2 Aziz,N., Paiva,N.L., May,G.D. and Dixon,R.A. Transcriptome analysis of alfalfa glandular trichomes. Planta 221 (1), 28-38 (2005).
REFERENCE 3 Li,X., Xia,Z., Wu,Y. and Li,H.Screening and analysis of alfalfa aluminum stress suppression subtractive library
Protein ref. UniProtKB:   Q40366
mRNA ref. GenBank:   L36157
EST ref. GenBank:   EY976961 [3' end]
Protein sequence: MsPrx15 (pxdC)
Sequence Properties
first value : protein
second value (mature protein)
Length (aa): %sProtein length in amino acid 353 (325)
PWM (Da): %sMolecular Weight of protein
Protein Mw is calculated by the addition of average isotopic masses
of amino acids in the protein and the average isotopic mass of one water molecule.
Molecular weight values are given in Dalton (Da).
38013.02 (35204.6) Transmb domain: %sTransmembrane domain
Calculation done with TMHMM
The topology is given as the position of the transmembrane helices
separated by i if the loop is on the inside or o if it is on the outside.
For example i7-29o44-66i87-109o means that it starts on the inside,
has a predicted TMH at position 7 to 29, the outside, then a TMH at position 44-66 etc.
i7-26o
PI (pH): %sIso electric point of protein
Calculation done with EMBOSS parameters.
Please see Dataset for pKi calculation for more information
6.02 (6.03) Peptide Signal: %sProtein signal for mature protein.
if exist :cut position of signal peptide.
range: start-end pos of mature protein
cut: 29 range:29-353
Sequence
Send to BLAST
Send to Peroxiscan
.........1 .........2 .........3 .........4 .........5 .........6 .........7 .........8 .........9 .........0 .........1 .........2
MNSSFNLTLA ALCCVVVVLG GLPFSSNAQL DNSFYRDTCP NVHSIVREVL RNVSKTDPRI LASLMRVHFH DCFVQGCDAS ILLNTTSTIT SEQTAFGNNN SIRGLDVVNQ IKTAVENACP  NTVSCADILA LAAEISSVLA HGPDWKVPLG RRDSLTANLT LANENLPSPA FNLSELKKNF DRQGLDTTDL VALSGAHTIG RGQCRFFVDR LYNFSNTGNP DPTLNTTYLQ TLRTICPNGG 
PGSTLTDLDP TTPDTFDSAY YSNLRIQKGL FESDQVLAST SGADTIAIVN SFNNNQTLFF EAFKASMIKM SKIKVLTGSQ GEIRKQCNFV NGNSGLATKV IRESSEDGIV SSY 

Retrieve as FASTA  
Remarks Complete sequence from mRNA and 3 ESTs. Cultivar="Siriver", "Xiao Guan".
CDS
Send to BLAST
.........1 .........2 .........3 .........4 .........5 .........6 .........7 .........8 .........9 .........0 .........1 .........2
ATGAACTCTT CTTTCAATCT CACATTGGCA GCTTTGTGCT GTGTAGTGGT TGTGCTTGGA GGGTTACCCT TCTCTTCAAA TGCACAACTT GATAATTCCT TCTATAGGGA CACCTGTCCT  AATGTTCATT CCATTGTTCG TGAAGTACTA AGGAATGTTT CTAAGACAGA TCCTCGTATA CTTGCTAGTT TGATGAGAGT TCACTTCCAT GATTGCTTTG TTCAAGGTTG TGATGCATCA  ATCTTGTTGA ATACCACTTC GACGATTACG AGTGAGCAAA CAGCTTTTGG AAATAACAAC TCAATAAGAG GTTTGGATGT TGTGAATCAA ATCAAAACAG CAGTTGAAAA TGCTTGTCCT  AACACTGTTT CTTGTGCTGA TATTCTTGCA CTTGCTGCTG AAATTTCCTC TGTTCTGGCT CATGGTCCTG ATTGGAAAGT GCCATTAGGA AGAAGAGATA GTTTAACAGC AAATTTAACC  CTTGCTAATG AAAATCTTCC TAGTCCCGCT TTCAACCTTA GTGAACTGAA AAAAAACTTT GATAGACAAG GTCTGGACAC TACAGATCTA GTTGCACTTT CAGGTGCTCA TACAATTGGT  AGAGGTCAAT GCAGATTCTT CGTTGATCGA TTATACAATT TCAGCAATAC TGGAAATCCC GATCCAACTC TCAACACAAC CTACTTACAA ACATTGAGAA CAATATGTCC CAATGGTGGA  CCTGGTAGCA CCCTCACTGA TTTGGATCCA ACCACTCCAG ATACATTCGA CTCGGCCTAC TATTCAAACC TAAGGATTCA AAAAGGCTTG TTTGAGAGTG ACCAAGTATT GGCTTCCACA  AGTGGCGCGG ACACGATTGC CATTGTCAAT AGTTTCAACA ACAACCAAAC TCTTTTCTTT GAAGCTTTTA AGGCCTCAAT GATTAAAATG AGTAAAATTA AGGTGTTAAC TGGATCTCAA 
GGTGAAATTA GAAAACAATG TAACTTTGTT AATGGAAATT CTGGTCTTGC CACTAAAGTC ATCAGAGAGT CGTCAGAGGA TGGTATTGTT AGCTCATACT AA 

Retrieve as FASTA  
cDNA
Send to BLAST
.........1 .........2 .........3 .........4 .........5 .........6 .........7 .........8 .........9 .........0 .........1 .........2
CAAAACAAGA GAAAAATAAT GAACTCTTCT TTCAATCTCA CATTGGCAGC TTTGTGCTGT GTAGTGGTTG TGCTTGGAGG GTTACCCTTC TCTTCAAATG CACAACTTGA TAATTCCTTC  TATAGGGACA CCTGTCCTAA TGTTCATTCC ATTGTTCGTG AAGTACTAAG GAATGTTTCT AAGACAGATC CTCGTATACT TGCTAGTTTG ATGAGAGTTC ACTTCCATGA TTGCTTTGTT  CAAGGTTGTG ATGCATCAAT CTTGTTGAAT ACCACTTCGA CGATTACGAG TGAGCAAACA GCTTTTGGAA ATAACAACTC AATAAGAGGT TTGGATGTTG TGAATCAAAT CAAAACAGCA  GTTGAAAATG CTTGTCCTAA CACTGTTTCT TGTGCTGATA TTCTTGCACT TGCTGCTGAA ATTTCCTCTG TTCTGGCTCA TGGTCCTGAT TGGAAAGTGC CATTAGGAAG AAGAGATAGT  TTAACAGCAA ATTTAACCCT TGCTAATGAA AATCTTCCTA GTCCCGCTTT CAACCTTAGT GAACTGAAAA AAAACTTTGA TAGACAAGGT CTGGACACTA CAGATCTAGT TGCACTTTCA  GGTGCTCATA CAATTGGTAG AGGTCAATGC AGATTCTTCG TTGATCGATT ATACAATTTC AGCAATACTG GAAATCCCGA TCCAACTCTC AACACAACCT ACTTACAAAC ATTGAGAACA  ATATGTCCCA ATGGTGGACC TGGTAGCACC CTCACTGATT TGGATCCAAC CACTCCAGAT ACATTCGACT CGGCCTACTA TTCAAACCTA AGGATTCAAA AAGGCTTGTT TGAGAGTGAC  CAAGTATTGG CTTCCACAAG TGGCGCGGAC ACGATTGCCA TTGTCAATAG TTTCAACAAC AACCAAACTC TTTTCTTTGA AGCTTTTAAG GCCTCAATGA TTAAAATGAG TAAAATTAAG  GTGTTAACTG GATCTCAAGG TGAAATTAGA AAACAATGTA ACTTTGTTAA TGGAAATTCT GGTCTTGCCA CTAAAGTCAT CAGAGAGTCG TCAGAGGATG GTATTGTTAG CTCATACTAA 
GTAATTTTAA GACAAGTTGC ATAAGAAAAT AAAGAGTGTG TTCTAGAGGC ACATACATGT TGTGCTCTTA TGGTGGATGG CGATAGGTT 

Retrieve as FASTA