Haloperoxidase superfamily

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Last update: 2018-06-08 (Bruno Savelli)

Haloperoxidase (haem) (HalPrx) No haem, no metal haloperoxidase (HalNPrx) No haem, Vanadium haloperoxidase: Bromoperoxidase (VBPo) , Chloroperoxidase (VCPo) , Iodoperoxidase (VIPo)

Profiles

Haloperoxidase Pfam : PF01328
Peroxidase
family 2. InterPro : IPR000028
Chloroperoxidase. No haem
no metal haloperoxidase
Pfam: PF00561
alpha/beta hydrolase fold. Interpro: IPR003089
Alpha/beta hydrolase; IPR000073
Alpha/beta hydrolase fold-1 and IPR000639
Epoxide hydrolase. No haem
Vanadium haloperoxidase
Pfam: PF01569
phosphatidic acid phosphatase domain (PAP2 superfamily). Interpro: IPR008934 and IPR003832
Acid phosphatase/vanadium-dependent haloperoxidase related; IPR000326
Phosphoesterase
PA-phosphatase related and IPR006162
phosphopantetheine attachment site.

Description

Haloperoxidases catalyzes the oxidative transformation of halides (Cl2, Br2 or I2) to XO- (X can be Cl, Br or I), or organic halogen compounds in the presence of hydrogen peroxide. Haloperoxidases catalyze the oxidation of halides, and they are named according to the most electronegative halide that they can oxidize; chloroperoxidases can catalyze the oxidation of chloride, as well as of bromide and iodide, bromoperoxidases react with bromide and iodide, whereas iodoperoxidases are specific of iodide.
The ability of haloperoxidases to halogenate a broad range of organic compounds of both commercial and pharmaceutical interest, as well as their high stability toward high temperatures, oxidative conditions, and in the presence of organic solvents, makes them good candidates for use in industrial biotransformations.


Tree classes of haloperoxidases have detected based on the presence or abscence of haem (replaced or no by metal). The amino acid sequence identities between the different Haloperoxidase classes are low.


HalPrx HalPrx are hemeproteins which contain contain protoporphyrin IX as a prosthetic group. HalPrx is also called chloroperoxidase (CPO). This versatile heme-containing enzyme that exhibits peroxidase, catalase and cytochrome P450-like activities in addition to catalyzing halogenation reactions. HalPrx acts on Cl-, Br-, and I-. Despite functional similarities with other heme enzymes, HalPrx folds into a novel tertiary structure dominated by eight helical segments. The catalytic base, required to cleave the peroxide O-O bond, is glutamic acid rather than histidine as in other peroxidases. They are only found in fungi (Ascomycetes and Basidiomycetes) with the exception of 3 sequences coming from Phytophthora. Catalyzes peroxidative halogenations involved in the biosynthesis of clardariomycin (2,2-dichloro-1,3-cyclo- pentenedione). The enzyme also has potent catalase activity and in the absence of halide ion, acts as a peroxidase similar to plant peroxidases.


HalVPrx Some haloperoxidase are non-haem and contain vanadium instead of haem. HalVPrx require vanadium in the form of vanadate as cofactor for the enzyme activity. HalVPrx can be chloroperoxidase (vCPO), bromoperoxidase (vBPO) and iodoperoxidase (vIPO). HalVPrx are detected in brown and red algae, in bacteria, in cyanobacteria and in some ascomycetes.


HalNPrx HalNPrx are non haem, chloroperoxidases enzymes. They are only found in procakyotes (bacteria)

Literature

Ohshiro T, Littlechild J, Garcia-Rodriguez E, Isupov MN, Iida Y, Kobayashi T, Izumi Y. Modification of halogen specificity of a vanadium-dependent bromoperoxidase. Protein Sci. 2004 Jun;13(6):1566-71. Epub 2004 May 7. PMID: 15133166 Bantleon R, Altenbuchner J, van Pee KH. Chloroperoxidase from Streptomyces lividans: isolation and characterization of the enzyme and the corresponding gene. J Bacteriol. 1994 Apr;176(8):2339-47. PMID: 8157602 Leblanc C, Colin C, Cosse A, Delage L, La Barre S, Morin P, Fievet B, Voiseux C, Ambroise Y, Verhaeghe E, Amouroux D, Donard O, Tessier E, Potin P. Iodine transfers in the coastal marine environment: the key role of brown algae and of their vanadium-dependent haloperoxidases. Biochimie. 2006 Nov;88(11):1773-85. PMID: 17007992 Colin C, Leblanc C, Wagner E, Delage L, Leize-Wagner E, Van Dorsselaer A, Kloareg B, Potin P. The brown algal kelp Laminaria digitata features distinct bromoperoxidase and iodoperoxidase activities. J Biol Chem. 2003 Jun 27;278(26):23545-52. PMID: 12697758 Pelletier I, Altenbuchner J, Mattes R. A catalytic triad is required by the non-heme haloperoxidases to perform halogenation. Biochim Biophys Acta. 1995 Jul 19;1250(2):149-57. PMID: 7632719