Entry information : PcCIIBA01 (PcNOP01 / nop-a / nop-b)
Entry ID 2332
Creation 2007-02-07 (Christophe Dunand)
Last sequence changes 2011-11-24 (Christophe Dunand)
Sequence status complete
Reviewer Christophe Dunand
Last annotation changes 2015-04-29 (Christophe Dunand)
Peroxidase information: PcCIIBA01 (PcNOP01 / nop-a / nop-b)
Name (synonym) PcCIIBA01 (PcNOP01 / nop-a / nop-b)
Class Other class II peroxidase type A    [Orthogroup: CIIBA001]
Taxonomy Eukaryota Fungi Basidiomycota Agaricomycetes Corticiaceae Phanerochaete
Organism Phanerochaete chrysosporium    [TaxId: 5306 ]
Cellular localisation N/D
Tissue type N/D
Inducer N/D
Repressor N/D
Best BLASTp hits
Perox score E-value PcCIIBA01
start..stop
S start..stop
PgigCIIBA01 323 3.98e-111 8..312 5..310
CuniCIIBA02 319 9.19e-109 5..311 3..309
CuniCIIBA03 317 9.38e-108 4..312 8..324
DsCIIBA06 313 3.58e-106 4..311 2..310
Gene structure Fichierperl './assets/cgi-bin/draw_exon.pl' '2332' 'join(1250082..1250201,1250258..1250301,1250354..1250387,1250443..1250456,1250504..1250548,1250600..1250613,1250668..1250706,1250760..1250838,1250894..1251134,1251201..1251254,1251334..1251449,1251500..1251528,1251583..1251701)' Exons
ExonStart..EndSize ExonStart..EndSize ExonStart..EndSize ExonStart..EndSize
N° 1 1250082..1250201 118 N° 2 1250258..1250301 42 N° 3 1250354..1250387 32 N° 4 1250443..1250456 12
N° 5 1250504..1250548 43 N° 6 1250600..1250613 12 N° 7 1250668..1250706 37 N° 8 1250760..1250838 77
N° 9 1250894..1251134 239 N° 10 1251201..1251254 52 N° 11 1251334..1251449 114 N° 12 1251500..1251528 27
N° 13 1251583..1251701 117  
join(1250082..1250201,1250258..1250301,1250354..1250387,1250443..1250456,1250504 ..1250548,1250600..1250613,1250668..1250706,1250760..1250838,1250894..1251134,12 51201..1251254,1251334..1251449,1251500..1251528,1251583..1251701)


exon

Literature and cross-references PcCIIBA01 (PcNOP01 / nop-a / nop-b)
Literature REFERENCE 1 Larrondo,L.F., Gonzalez,A., Perez-Acle,T., Cullen,D. and Vicuna,R. The nop gene from Phanerochaete chrysosporium encodes a peroxidase with novel structural features. Biophys Chem 2005 Jul 1, 116(2): 167-173.
REFERENCE 2 Martinez,D., Larrondo,L.F., Putnam,N., Sollewijn Gelpke,M.D., Huang,K., Chapman,J., Helfenbein,K.G., Ramaiya,P., Detter,J.C., Larimer,F., Coutinho,P.M., Henrissat,B., Berka,R., Cullen,D. and Rokhsar,D. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nat. Biotechnol. 22 (6), 695-700 (2004).
Protein ref. UniProtKB:   Q5XXE3  Q5XXE5 [Mismatch]  Q5XXE2 [Other strain]
DNA ref. JGI genome:   scaffold_10 (1250082..1251701)
mRNA ref. GenBank:   AY727765
Protein sequence: PcCIIBA01 (PcNOP01 / nop-a / nop-b)
Sequence Properties
first value : protein
second value (mature protein)
Length (aa):   %s   315 (295)
PWM (Da):   %s   33528.94 (31349.7) Transmb domain:   %s   i7-24o
PI (pH):   %s   6.9 (6.67) Peptide Signal:   %s   cut: 21 range:21-315
Sequence
Send to BLAST
Send to Peroxiscan
.........1.........2.........3.........4.........5.........6.........7.........8.........9.........0.........1.........2
MHNLIKVIYILIIATVAAMAAPPSPNRVRCSAGREAGHPECCKWFDVREEIQDRLFNRSCGADVRSIRIQWHDAIAFSHTGGLKSGGGDGSILAHPIELAYPANVGLSDIAERQRALALKHNVSFGDFIQFASAVGLSNCEGAPRLTFLAGRPNASRAAD
DGLVPAPWHSADTLLARMADAGFAPHELVALLAGHSVATQQTVDASV
GTPLDSTPRTFDNAFFVETQARGTVCPGAALHKGEALSSSPHELRLQSDGALARDPRTAPTWKRFEDQVEMAR
QFANAMAKLSVVGQDAASLVDCTDVVPRWRM

Retrieve as FASTA  
Remarks Complete sequence from genomic and mRNA. Strain="BKM1767", "RP-78" and "ME446". No EST. The 3'end looks shorter with missing conserved residues.
This class II peroxidase has been called "NoP", i.e. "novel peroxidase", by its discoverers. It is considerably shorter than other class II peroxidases. Two SwissProt Q5XXE3 (nop-b) and Q5XXE5 (nop-a) submitted by the same authors (differs by a few amino acids). There is only ONE nop gene in the P.chrysosporium genome (BLAST in Joint Genome Institute).
DNA
Send to BLAST
.........1.........2.........3.........4.........5.........6.........7.........8.........9.........0.........1.........2
ATGCACAACTTAATCAAGGTAATATACATTCTTATCATAGCGACGGTGGCTGCTATGGCTGCACCTCCGTCGCCGAATCGGGTCCGGTGTAGCGCAGGCCGCGAGGCAGGACATCCCGAG
AGGTGGGGCAAGCGTGCCGCGAGACAGTCGCCACGGGGAACTCACCTTGCACGCGCAGTGTTGCAAGTGGTTCGACGTCCGGGAGGAGATACAAGACAGGCTCTGTAAGCAGGTGCAGCG
TCGTCCACTCGAGTCCTCGATACTGAACGCGCACAGGTTCAACCGGTCGTGTGGTGCCGACGTCCGGAAGAGGTGCGACCAGCGTACAATGCTTGTTGCGACGGATCCTTGCTGACGTGG
CGTCTAGTCGATCCGTATCCACAGTGAGCCCAGTGTGTCTCTGGTCCCGTCCCGGTCCTAAAGTCCGCAGGTGGCACGACGCCATCGCCTTCTCACACACGGGGGGATTGAAGAGAGGTA
CTGTCCAAGCATCTCCTAGCGTTCTGCCTTCTTTCTGAATATGAGCAGCGGTGGGGGCGCAGAGGTGCGTGATCCGTGTCTTACTCTCACAGCATTTGTTCTGAACGCATATATGCAGAC
GGCTCTATCCTCGCACATCCCATCGAACTAGCCTACC
CCGTGAGCGACACCGTTCTCAACTAGCGCAAGCACACCCTGATACTCTGGTACAGCGGCGAATGTAGGCCTGTCCGACATCGC
AGAGCGCCAGCGCGCCCTCGCCCTGAAGCACAACGTATCCTTTGGCGACTT
TTGTGCGCGGATCCGCTGCTCCCACGCAGGTGCACCATCTGACGCCGTACCTTGCAGCATACAATTTGC
AAGCGCCGTTGGGCTATCAAACTGCGAGGGTGCACCACGCCTGACGTTTCTCGCGGGACGGCCGAACGCCTCGCGAGCTGCGGACGACGGGCTGGTCCCCGCTCCATGGCACTCCGCCGA
CACCCTCTTGGCGCGGATGGCCGACGCCGGATTTGCCCCCCACGAGCTCGTCGCGCTGCTTGCGGGCCACAGCGTTGCGACCCAGCAAACGGTCGACGCGAGCGTCGCG
CGGTGCGTGAC
ACATCGACGATTCCCGCGTCGTCTTTGTCTTCGCGCTCATGGTGCCTGCTGTTGCAGGGCACGCCGCTGGATTCGACACCGCGCACGTTCGACAACGCGTTCTTTGTCGAGAGGTGAGCG
TGTCCTGCCAGAGACGCGCGAGGCGCAGATCGGCCGGGCGCTGATGACGGTTCCGTGCATGCCCCCCCTCAGACGCAGGCCAGAGGAACGGTCTGTCCCGGCGCTGCGCTGCACAAGGGC
GAGGCCCTATCGTCCTCGCCCCACGAGCTGCGACTACAGTCCGATGGCGCGCTCGCTCGAGATCCCAG
AGGTGCGTGTGCGCGACGTGGACCGCCTTGTCAAGACTGATGGACATGTCAG

GACCGCCCCGACATGGAAACGGTTTGCAG
AGGTGCGCTCAGGCCTCCCTCCGAGACTCCATACACATCTGAAGGTGTCCCTGCAGAGGATCAGGTCGAGATGGCAAGGCAGTTTGCGAAC
GCGATGGCGAAGCTGTCTGTCGTGGGTCAGGATGCAGCCTCGCTGGTCGACTGCACGGACGTCGTCCCTCGATGGAGAATGTAA

Retrieve as FASTA  
CDS
Send to BLAST
.........1.........2.........3.........4.........5.........6.........7.........8.........9.........0.........1.........2
ATGCACAACTTAATCAAGGTAATATACATTCTTATCATAGCGACGGTGGCTGCTATGGCTGCACCTCCGTCGCCGAATCGGGTCCGGTGTAGCGCAGGCCGCGAGGCAGGACATCCCGAG
TGTTGCAAGTGGTTCGACGTCCGGGAGGAGATACAAGACAGGCTGTTCAACCGGTCGTGTGGTGCCGACGTCCGGAAGTCGATCCGTATCCAGTGGCACGACGCCATCGCCTTCTCACAC
ACGGGGGGATTGAAGAG
CGGTGGGGGCGCAGACGGCTCTATCCTCGCACATCCCATCGAACTAGCCTACCCGGCGAATGTAGGCCTGTCCGACATCGCAGAGCGCCAGCGCGCCCTCGCC
CTGAAGCACAACGTATCCTTTGGCGACTT
CATACAATTTGCAAGCGCCGTTGGGCTATCAAACTGCGAGGGTGCACCACGCCTGACGTTTCTCGCGGGACGGCCGAACGCCTCGCGAGCT
GCGGACGACGGGCTGGTCCCCGCTCCATGGCACTCCGCCGACACCCTCTTGGCGCGGATGGCCGACGCCGGATTTGCCCCCCACGAGCTCGTCGCGCTGCTTGCGGGCCACAGCGTTGCG
ACCCAGCAAACGGTCGACGCGAGCGTCGCG
GGCACGCCGCTGGATTCGACACCGCGCACGTTCGACAACGCGTTCTTTGTCGAGACGCAGGCCAGAGGAACGGTCTGTCCCGGCGCTGCG
CTGCACAAGGGCGAGGCCCTATCGTCCTCGCCCCACGAGCTGCGACTACAGTCCGATGGCGCGCTCGCTCGAGATCCCAG
GACCGCCCCGACATGGAAACGGTTTGCAGAGGATCAGGTC
GAGATGGCAAGGCAGTTTGCGAACGCGATGGCGAAGCTGTCTGTCGTGGGTCAGGATGCAGCCTCGCTGGTCGACTGCACGGACGTCGTCCCTCGATGGAGAATGTAA

Retrieve as FASTA