NADH peroxidase (NadPrx)
NADH oxidase (NadOxd)
NADH dehydrogenase (NadDH)
Pfam: PF00070
Pyr_redox; PF07992
Pyr_redox_2; PF02852
Pyr_redox_2 dimerisation domain.
Interpro: IPR001100
Pyr_redox; IPR013027
FAD_pyr_redox; IPR001327
Pyr_redox_NAD_bd; IPR004099
Pyr_redox_dim
Strong sequence homology exists between NADH peroxidase (NadPrx) and NADH oxidase (NadOxd) which can suggest that both genes originally diverged from a common ancestral sequence. NadPrx adn NadOxd share low level of identity with other members of the flavoprotein disulfide reductase classes. NadPrx as well as other pyridine nucleotide-disulphide reductases (PNDR) use the isoalloxazine ring of FAD to shuttle reducing equivalents from NAD(P)H to a Cys residue that is usually a part of a redox-active disulphide bridge. In a second step, the reduced disulphide reduces the substrate. Particular residues have been found to be highly conserved in within the FAD/NAD binding domain.
NADH peroxidase NADH + H+ +H2O2 → 2 H2O + NAD+
NADH oxidase
1) 2 NADH + 2 H+ +O2 → 2 H2O + 2 NAD+
2) NADH + H+ +H+ + O2 → H2O2 + NAD+
3) NADH + 2O2 → H+ + NAD+ + 2O2.
NADH dehydrogenase
Ross RP, Claiborne A. Molecular cloning and analysis of the gene encoding the NADH oxidase from Streptococcus faecalis 10C1. Comparison with NADH peroxidase and the flavoprotein disulfide reductases. J Mol Biol. 1992 Oct 5;227(3):658-71. PMID: 1404382
Parsonage D, Miller H, Ross RP, Claiborne A.Purification and analysis of streptococcal NADH peroxidase expressed in Escherichia coli. J Biol Chem. 1993 Feb 15;268(5):3161-7. PMID: 8428993
Yeh JI, Claiborne A, Hol WG. Structure of the native cysteine-sulfenic acid redox center of enterococcal NADH peroxidase refined at 2.8 A resolution. Biochemistry. 1996 Aug 6;35(31):9951-7. PMID: 8756456
Lountos GT, Jiang R, Wellborn WB, Thaler TL, Bommarius AS, Orville AM. The crystal structure of NAD(P)H oxidase from Lactobacillus sanfranciscensis: insights into the conversion of O2 into two water molecules by the flavoenzyme. Biochemistry. 2006 Aug 15;45(32):9648-59. PMID: 16893166